People coming to the UK from France and the Netherlands will be forced to quarantine for 14 days from Saturday.
Transport Secretary Grant Shapps said the measure – which also applies to people travelling from Monaco, Malta, Turks and Caicos, and Aruba – would kick in from 04:00 BST.
He added that this was necessary to keep coronavirus infections down.
France warned the UK decision would lead to “reciprocal measures” across the Channel.
Clement Beaune, France’s secretary of state for European affairs,tweeted that the UK’s decision was a matter of “regret” for the French.
The UK government’s decision follows a surge in cases in the countries affected in recent days.
Airlines UK described it as “another devastating blow to the travel industry already reeling from the worst crisis in its history”.
With up to half a million UK tourists thought to be in France at present, the deadline is expected to induce a rush to ports and airports, with thousands of tourists desperate to avoid quarantine.
The Eurotunnel website is reportedly struggling to deal with the volume of inquiries.
John Keefe, director of public affairs at Getlink, which operates the Channel Tunnel, told BBC’s Newsnight programme that the trains were “already pretty much fully booked” on Friday.
He said: “We just haven’t got the space to take everybody who might suddenly want to come up to the coast.”
Urging travellers to check online if there is space for them before heading to the terminal, he said people should understand “that it’s not going to be easy to get back” to the UK.
The UK’s ambassador to France, Lord Llewellyn,acknowledged that the new quarantine rule would be “unwelcome news” for Britons in the country, but stressed that people could continue with their holidays as long as they follow safety precautions and self-isolate on their return.
Shadow home secretary Nick Thomas-Symonds said while the Labour party supports “evidence based measures” at the border, it was “vital” that No 10 had a “joined-up strategy” and “urgently” puts in place a specific deal to support the heavily impacted travel sector.
The MP added: “That the government has still not put in place an effective track, trace and isolate system has made matters far worse and made it more li